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High spatial variability of phytoplankton assessed by flow cytometry, in a dynamic productive coastal area, in spring:the eastern English Channel ArchiMer
Bonato, Simon; Christaki, Urania; Lefebvre, Alain; Lizon, Fabrice; Thyssen, Melilotus; Artigas, Luis Felipe.
The distribution of phytoplankton (from pico-to microphytoplankton) was investigated, at single-cell level and at high spatial resolution, during an oceanographic cruise across the eastern English Channel (EEC) between April 27 and 29, 2012. Seawater was continuously collected from surface waters and analysed on board at high frequency (one sample every 10 min), by using a new generation of pulse-shape recording scanning flow cytometer (CytoSense, Cytobuoy©). A Bray-Curtis matrix analysis based on phytoplankton composition allowed the discrimination of 4 communities. Within these communities, abundance, cell size as well as single cell and total red fluorescence of 8 phytoplankton groups were measured. Picoeukaryotes and Synechococcus spp. cells dominated...
Tipo: Text Palavras-chave: Phytoplankton dynamics; High spatial resolution; Flow cytometer; Red fluorescence.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00248/35885/34406.pdf
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Modulation des paramètres hémocytaires par la nutrition de l'huitre creuse Crassostrea gigas. Implication dans les mortalités estivales ArchiMer
Delaporte, Maryse.
In the context of Pacific oyster C. gigas summer mortality, the present study demonstrated that the reproductive effort reduced not only the energetic parameters (carbohydrates and CEA) but also the immune parameters (concentration, phagocytosis and adhesive capacity of hemocytes). Increasing food supply from 4% to 12% (of oyster dry weight) did not counteract this phenomena as most of the additional energy was dedicated to the gametogenesis processes. However, after the gametogenesis, the 12% diet clearly favored energy storage restoration and hemocyte production. Hemocyte parameters were also modulated by the food quality. Indeed, hemocyte concentration tended to increase 20:5n-3 and/or 20:4n-6 were supplied by micro-algae or artificial diets. When these...
Tipo: Text Palavras-chave: Summer mortalities; Flow cytometer; Immunity; Oxidative burst; Phagocytosis; Biochemical composition hemocyte parameters; Essential fatty acids; Nutrition; Crassostrea gigas; Mortalités estivales; Cytomètre de flux; Immunité; Poussée respiratoire; Phagocytose; Réponses hémocytaires; Composition biochimique; Acides gras essentiels; Nutrition; Crassostrea gigas.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-357.pdf
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